Phyto@Wiki
Procedure
最終更新:
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DNA extraction
↓ ↓
↓ ・Agarose E.P. Check
↓ ・Quantification of DNA
↓
↓ ・Agarose E.P. Check
↓ ・Quantification of DNA
↓
PCR for 1st DGGE
↓
↓ → Agarose E.P. Check
↓
↓ → Agarose E.P. Check
↓
1st DGGE
| ↓
| Get DGGE pattern → Anaysis of DGGE pattern
| ↓
| Band Excision
| ↓
| Get DGGE pattern → Anaysis of DGGE pattern
| ↓
| Band Excision
| ↓
PCR for 2nd DGGE
↓
↓ → Agarose E.P. Check
↓
↓ → Agarose E.P. Check
↓
2nd DGGE
↓
Purify PCR products
↓
Cycl-sequencing
↓